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Virus titration by plaque assay

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Download : Virus titration by plaque assay(2).hwp





9. Overlay(2XDMEM 10ml + 2% agar + trypsin 80ul)를 제조한다.
5. 6 well plate를 PBS로 washing 해 준다.

4. 6 well plate에 10-1 부터 10-6까지 적어준다.
7. 45분 동안 실온에서 기다린다.

Virus titration by plaque assay
순서
실험결과/생물의학


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Virus,titration,by,plaque,assay,생물의학,실험결과


Virus titration by plaque assay
Virus titration by plaque assay , Virus titration by plaque assay생물의학실험결과 , Virus titration by plaque assay




Title
Virus titration by plaque assay
Date
2015/4/7/Tue
Objective
1. Introduction of plaque assay which is most widely used for titration of influenza
virus particles
Material
1. Cell preparation
6 well plate, MDCK cell Serumfree MEM, PBS
2. Plaque assay for virus titration
Virus stock solution, Trypsin, 2XDMEM, 2% low-melting agar, pipette
filter tip, e-tube, micro tube
3. Plaque staining
4%(v/v)formalin solution
1%(w/v)crystal violet staining solution
Method
1. 하루 전...



Title
Virus titration by plaque assay
Date
2015/4/7/Tue
Objective
1. Introduction of plaque assay which is most widely used for titration of influenza
virus particles
Material
1. Cell preparation
6 well plate, MDCK cell Serumfree MEM, PBS
2. Plaque assay for virus titration
Virus stock solution, Trypsin, 2XDMEM, 2% low-melting agar, pipette
filter tip, e-tube, micro tube
3. Plaque staining
4%(v/v)formalin solution
1%(w/v)crystal violet staining solution
Method
1. 하루 전, 6 well plate에 MDCK cell을 106 culture를 만들어 둔다.
3. virus 100ul을 섞어 10-1 sample을 만들고 10-1에서 100ul을 따서 10-2에 넣어주어 10-2
sample을 만드는 과정을 모두 해준다.
8. 6 well plate를 PBS로 다시 washing 해 준다.
11. 37℃ 배양 3일후…(drop)

Download : Virus titration by plaque assay(2).hwp( 21 )


Virus%20titration%20by%20plaque%20assay(2)_hwp_01.gif Virus%20titration%20by%20plaque%20assay(2)_hwp_02.gif





다.
10. 제조한 Overlay를 3ml씩 6 well plate에 넣어준다.
2. micro tube 10-1 부터 10-6까지 적어준 후 micro tube MEM 900ul을 각각 넣어준다.
6. 6 well plate에 virus를 각각 넣는다.

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